Colder Products” HFC39 Series couplings have been tested for biocompatibility and performance and are designed to prevent external organisms from entering through the connector and into the flow path during disconnection. When integrated into pre-sterilized single-use systems, the coupling”s automatic shutoff valves allow an aseptic disconnection by closing off the flow path prior to fully disconnecting. This allows users to complete an aseptic break between silicone tubing assemblies. The valve design also eliminates the need for pinch clamps or tubing welders. The plastic components of the couplings are made from polysulfone, and the seals are made from platinum-cured silicone, which meets U.S.P. Class VI standards and will withstand autoclave, EtO, Gamma, or E-Beam sterilization methods. The couplings function reliably with working pressure from vacuum to 125 PSI (8.6 bar), and in temperatures from -40 F to 280 F (-40 C to 138 C). The couplings have a 3/8″ (9.5 mm) diameter flowpath and are available with hose barbs for 1/4”, 3/8″ and 1/2″ (6.4mm, 9.5mm & 12.7mm) tubing.